description
specification
resources
service description
modern liquid chromatography and mass spectrometry technology provides a means to routinely perform non-targeted protein identification¹.
bgi can help simplify the challenge of protein identification for your project.
project workflow
in-gel identification
we can identify proteins isolated in gel bands or spots. our service includes destaining, in-gel digestion, nano-flow lc-ms/ms, and peptide spectral match data analysis.
- in-gel digestion
- lc-ms/ms analysis
- data analysis
in-solution identification
our in-solution protein identification services can be used for applications such as monitoring recombinant protein expression and affinity purification systems.
- in-solution digestion
- lc-ms/ms analysis
- data analysis
references
[1]. baldwin ma. protein identification by mass spectrometry: issues to be considered. mol cell proteomics. 2004 jan;3(1):1-9. epub 2003 nov 6. review. pubmed pmid: 14608001
how to order
mass spectrometry service specification
protein identification services are performed using nano-flow liquid chromatography and high resolution orbitrap mass spectrometry.
sample preparation and services
- digestion performed using sequencing-grade trypsin
- 60 min nano-flow lc-ms/ms using a q exactive hf-x orbitrap mass spectrometer
mass spec services quality standard
- summary including all methods and data analysis
- reports provided in excel or pdf format, raw files available upon request
turn around time
- typical 2-3 weeks from sample qc acceptance to data report delivery
- prime
expedited services with 1 week turn around time is available
sample requirements
we accept protein samples in solution or gel format. for complex in-solution samples we can provide electrophoresis services, including preparative sds-page and analytical staining.
| sample type | amount and concentration | minimum sample volume |
|---|---|---|
| protein sample in liquid solution or gel band/spot | recommended 100 µg; 2 µg/µl or above | 10 µl |
data analysis
advanced bioinformatics
- data analysis and validation performed with sequest or mascot
- top protein identifications, peptide spectral match count, and sequence coverage provided
- up to 30 proteins ranked by peptide spectral match count