description
specification
resources
project workflow
dda analysis can be carried out if there are no suitable library construction samples:
- protein extraction
- protein digestion
- individual samples
- lc-ms/ms (dda)
dia analysis can be carried out if there are suitable library construction samples:
references
[1] kleiner m. metaproteomics: much more than measuring gene expression in microbial communities. msystems. 2019 may-jun; 4(3): e00115-19.
how to order
mass spectrometry service specification
metaproteomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (q exactive hf/hf-x and orbitrap fusion lumos).
sample preparation and services
- the qc analysis of enzymatic hydrolysis is strictly controlled by lc-ms
- dda-spectral library generated using hplc technology and sample fractionation
quality standard
- summary includes all methods and data analysis
- reports provided in excel or pdf format, raw files available upon request
turn around time
- typical 4-5 weeks from sample qc acceptance to data report delivery
data analysis
data analysis
- data analysis and validation performed with mascot
- protein analysis
- species analysis
- diversity analysis
- function analysis
- correlation analysis